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MXMoS 2 DNAgel effectively accelerates bone regeneration and suppresses infection in pyogenic osteomyelitis. (A) Schematic diagram of the treatment procedure of suppurative osteomyelitis mice. (B) Bacterial colonies in mice wounds before and after NIR treatment. (C) Quantification of bacterial colony counts. (D) 3D micro-CT reconstruction of maxilla at 4 weeks after different treatment. Representative images of H&E and Masson's trichrome staining of the maxilla at 4 weeks treatment. Immunofluorescence staining for IL-6 (red), TRAP (red) and OCN (red) in maxilla. Nuclei were counterstained with DAPI (blue). (E) BMD and (F) BV/TV analyses of newly formed bone calculated based on micro-CT. (G – I) Quantitative analysis of the ROI. Data are presented as mean ± SD (n = 5 mice per group). ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

Journal: Bioactive Materials

Article Title: Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS 2 in the treatment of pyogenic osteomyelitis

doi: 10.1016/j.bioactmat.2025.10.023

Figure Lengend Snippet: MXMoS 2 DNAgel effectively accelerates bone regeneration and suppresses infection in pyogenic osteomyelitis. (A) Schematic diagram of the treatment procedure of suppurative osteomyelitis mice. (B) Bacterial colonies in mice wounds before and after NIR treatment. (C) Quantification of bacterial colony counts. (D) 3D micro-CT reconstruction of maxilla at 4 weeks after different treatment. Representative images of H&E and Masson's trichrome staining of the maxilla at 4 weeks treatment. Immunofluorescence staining for IL-6 (red), TRAP (red) and OCN (red) in maxilla. Nuclei were counterstained with DAPI (blue). (E) BMD and (F) BV/TV analyses of newly formed bone calculated based on micro-CT. (G – I) Quantitative analysis of the ROI. Data are presented as mean ± SD (n = 5 mice per group). ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

Article Snippet: Primary antibodies for immunocytochemistry and immunofluorescence staining—including ARG1 and iNOS, TRAP, β-Catenin, OCN and WNT4—were obtained from Proteintech (China).

Techniques: Infection, Micro-CT, Staining, Immunofluorescence